Molecular and Seroepidemiological Survey of Visceral Leishmaniasis among Humans and Domestic Dogs in Mazandaran Province, North of Iran

نویسندگان

  • M Fakhar
  • B Rahmati
  • S Gohardehi
  • M Mohebali
  • B Akhoundi
  • M Sharif
  • S Ali Mahdavi
چکیده

BACKGROUND New cases of visceral leishmaniasis (VL) have been reported recently in some parts of Mazandaran Province, north of Iran where the first human case of VL was reported in 1949. This study aimed to determine the present status of Leishmaniainfantum infection among humans and domestic dogs using serological and molecular methods in central parts of Mazandaran Province. METHODS In this cross-sectional study, blood samples were randomly collected from 402 humans and forty-nine domestic dogs throughout 2009 and 2010 in the central part of Mazandaran Province including Semeskadeh and Kiakola districts where recent cases of human visceral leishmaniasis had been reported there. All the collected samples were tested by direct agglutination test (DAT) for the detection of anti-Leishmania infantum antibodies as well as convenience PCR assay on whole blood samples for detection of leishmanial infection and identification of Leishmania species. RESULTS None of 402 collected human (402) and dog (49) blood samples showed anti Leishmaniainfantum antibodies at titers 1:3200 and 1:320 as cut-off values of DAT, respectively but only 2 of domestic dogs (4.1%) were found PCR-positive corresponding to L.infantum. CONCLUSION This study confirms the circulation of L. infantum at least among domestic dogs and highlights the sporadic pattern of VL in the studied areas. Further investigations regarding to sand flies fauna and wild canines as reservoir hosts of the disease, are recommended.

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منابع مشابه

Dear Editor-in-Chief Iranian J Parasitol

I received Iranian Journal of Parasitology (Volume 6 (4), Oct-Dec 2011). I have seen an article in pages 51-58 titled "Molecular and Seroepidemiological Survey of Visceral Leishmaniasis among Humans and Domestic Dogs in Mazandaran Province, North of Iran ". I think this title needs to be revised, because this study was done in two limited districts of the central zone of Mazandaran (Semeskandeh...

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عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2011